The resource file below supports the paper "Massively Parallel Single-Cell RNA-Seq for Marker-Free Decomposition of Tissues into Cell Types" by Diego Adhemar Jaitin*, Ephraim Kenigsberg*, Hadas Keren-Shaul*, Naama Elefant, Franziska Paul, Irina Zaretsky, Alexander Mildner, Nadav Cohen, Steffen Jung, Amos Tanay†, Ido Amit†. science 2014.

Each row contain the mRNA counts of a gene per each well (columns). Column names specify the batch ID and well ID, delimited by underscore.

Note that for the clustering analyis presented in the paper, we exlcuded the following genes that show significant batch specifc expression: "M34473","abParts","M13680","Tmsb4x","S100a4","B2m","Atpase6","Rpl23","Rps18","Rpl13","Rps19","H2-Ab1","Rplp1","Rpl4","Rps26","EF437368"

Each row in this file specifies the barcodes, index, and experimental details regarding one well (usually occupied by a single cell).

Low level analysis will require the following data:

This tarball contains 28 fastq files that corresponds to biological samples (amplification batches) included in the paper. Reads can be associated with a single well/molecule by the last 3 fields of the read header (plate_barcode:well_barcode:RMT).

To go from raw fastq files to the demultiplexed version, we were only extracting plate,well and umi barcodes from Read2, and storing the information in the read 1 header. Subsequent statistical processing, as described in this paper, may still further eliminate reads based on demultiplexing errors or other biases.

Model green design squeezes most from tight city budget The new building creates an urban street presence at a suburban intersection. The tight budget and schedule requirements of a Design Build contract, combined with the sustainable agenda of a LEED municipal client set this project apart from others of similar scope and Replica Christian Louboutin scale. The four storey office building is an extension to the existing Thorold City Hall, and consolidates operational services for the Niagara Region. The building wraps around a triangular courtyard which acts as both a ventilation cheap michael kors outlet shaft and a communal lobby for the facility. This form creates a strong urban corner in a suburban context at the intersection of cheap gucci Thorold and St. David Road, with the entrance addressing the street rather than the parking lot. As a municipal building it was critical that cheap michael kors the project be realized as economically as possible and that it be a model of green design for the community. The building program consists of a variety of office facilities for municipal services,
MPP Van Bommel acknowledges As replica Michael Kors Ontarians, we are blessed to live in a province with a rich history and great diversity. It is one of our greatest strengths. A critical element of that history is the experience of the black community in Ontario, cheap gucci which is an integral part of the social, cultural and economic fabric of our society. Lambton Kent Middlesex has a rich history of diverse settlement as well. As a result of a Bill Michael Kors outlet I co sponsored with MPP Ted Arnott in 2008, Ontario now proclaims August 1st as Emancipation Day, under the Emancipation Act, 2008. February is Black History Month, an important opportunity for all Ontarians to pay tribute to the valuable contributions black Canadians have chi flat irons made to our province and country, and to celebrate the history, culture and heritage of the black community. It is a time to celebrate the people who, despite many historical barriers, helped build, develop, and defend this Christian Louboutin Online Store country. People like Anderson R. Abbott, the christian louboutin replica first Canadian born black doctor; or Leonard A. Braithwaite,
Articles Connexes：